Moreover, a set of genes, that are particularly expressed in pollen tubes in response to their development within the pistil but not expressed during different stages of pollen or plant improvement, was identified. For 33 pollen tube–expressed genes the respective mutants had been analyzed in a reverse genetics method. The mutants were derived from a subset of the SAIL collection that was generated within the quartet background containing a LAT52 promoter driven GUS reporter gene . Indeed, seven out of 33 investigated genes have been important for pollen tube growth .
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