This outcome highlights the need to use sturdy, high-throughput phenotypic screening methods to assign a phenotype, i.e. a biological function, to each gene, even in medium-scale screens. In addition, for genes that aren’t lined by current mutant collections, partial loss-of-function traces are sometimes generated by the knock-down strategy (see Section 2.2). Treatment of mutant assortment with a chemical compound to elucidate gene capabilities and signal transduction pathways are broadly utilized . Limitations of this strategy could be limited uptake of the compound by the plant or transport over membranes, and that possible modifications corresponding to acetylation might inactive the compound. A prerequisite for these so referred to as “chemical genetic screens” is the availability of a library of chemical compounds. The chemicals are normally used to display screen for modifications of a specific physiological parameter.